Fluorescence Experiment Simulators

Fluorescence experiments for chemists, biochemists, biologists, physicists and general scientists.

Let your students work their way through carefully designed experiments using a fluorescence lifetime spectrometer simulator to ultimately derive physical parameters for various molecular systems.

Arrhenius Behaviour

Arrhenius behaviour occurs in many systems in physical chemistry and even in everyday life. The Arrhenius equation relates the rate constant of a chemical reaction to temperature and the activation energy for the process (the energy barrier that must be overcome for reaction to proceed.) It’s why we store food in refrigerators: since rotting food is a chemical reaction we can slow the rate of decay down by storing in a cool environment. The fluorescence emission of certain aromatic molecules when dissolved in solution will also show an Arrhenius type thermal dependence. Our simulation software allows the student to prepare a luminescent solution and observe its fluorescence decay over the temperature range 20 ºC to 60 ºC via a fluorescence lifetime spectrometer. Ultimately, the rate constants for fluorescence and the activation energy for the process can be derived.

Fluorescence Experiment 1 Download – Arrhenius Behaviour – Free Evaluation

When you have downloaded and tried our simulator please take part in our anonymous survey.

Click here to take the survey

What do you get if you purchase the Arrhenius Experiment?

Single Photon Counter Simulator; Teaching Notes (Sim Pack 1-5); Experimental Procedure; Teacher’s / Supervisor’s Notes including model answers.

Stern-Volmer Quenching

Certain molecules (i.e., quenchers) can deactivate fluorescence from fluorophores in solution by collision. This process can, in some instances, be described by Stern-Volmer kinetics, which relates the efficiency of quenching at some concentration of quencher to the bimolecular rate constant. The rate at which the quencher diffuses through the medium is governed by the viscosity of the solvent. Consequently, Stern-Volmer quenching provides an experimental method to directly measure molecular diffusion rates. Our simulation software allows the student to prepare a fluorophore in two solvents of very different viscosity. The effect of viscosity on the quenching rate constant can then be assessed by measuring fluorescence decays as a function of quencher concentration in each solvent.

Fluorescence Experiment 2 Download – Stern-Volmer – Free Evaluation

Preview Documentation – Look Inside

Below are sample pages from our teaching documentation to give you a flavor for the quality of material supplied upon purchase of one of our experiments.

Sim Pack 1 Preview – Fluorescence

Sim Pack 2 Preview – Single Photon Counting

Sim Pack 3 Preview – Rate of Reaction and Arrhenius Behaviour

Sim Pack 4 Preview – UV-Vis Absorption Spectroscopy and the Beer Lambert Law

Sim Pack 5 Preview – Stern-Volmer Kinetics

Fluorescence Experiment 1 Preview – Arrhenius Behaviour

Fluorescence Experiment 2 Preview – Diffusion Controlled Reactions

System Requirements

Our simulators do not require the latest or the greatest computers. If you have a new PC then great but if you need to use an obsolete PC running Window-98 SE or later then, provided it can read a CD you can probably use it. Just give it a try – you have nothing to lose!

What to do next?

Simply download the fully functional “demonstration version” of our software for evaluation purposes.  If you wish to proceed to purchase a full licence for teaching purposes contact our sales department to arrange payment following which you will also receive additional documentation and other support material.

Enjoy the fun and tell your friends!!