UV-Vis absorption experiments for chemists, biochemists, biologists, physicists and general scientists.
Let your students work their way through carefully designed experiments using a UV-Vis absorption spectrometer simulator.
UV-Vis Absorption Spectroscopy
Ultraviolet-Visible (UV-Vis) spectroscopy is concerned with measurement of the interaction of outer shell electrons with electromagnetic radiation in the range 190 nm to 700 nm. Electrons of organic compounds with a high degree of conjugation or aromaticity, transition metal irons and charge transfer complexes are active in this part of the spectral region. UV-Vis spectroscopy has many uses across science such as in monitoring the progress of a chemical reaction, through analysis of pharmaceutical substances and in biology, in investigation of proteins. UV-Vis spectroscopy involves measurement of the fraction of incident electromagnetic radiation that is either absorbed or transmitted by a sample and this process can be described by the Beer-Lambert Law. One of the key features of this law is that the absorbance (the radiation that is absorbed by the sample) is related to the concentration of the absorbing species present which is important in analytical science and quantitative analyses.
UV-Vis absorption measurements can be performed at a single wavelength or across a broad range of wavelengths when a spectrum is produced. A spectrum can serve as a fingerprint for a compound which is of importance in identification of unknown materials (or qualitative analyses) but can also provide information concerning the degree of conjugation or aromaticity present in a sample.
UV-Vis Absorption Experiment 1: Beer Lambert Law & Identification of an Unknown Mixture
This experiment provides experience for students in quantitative and qualitative analysis. UV-Vis spectra will be recorded for several, simple aromatic molecules in toluene solution. Beer Lambert plots will be constructed for each aromatic species and the molar extinction coefficient determined. In the second part of the experiment, unknown samples will be investigated. Given the information derived in part one, UV-Vis spectroscopy will be used qualitatively to identify the species, and then quantitatively to determine the amount of each component present in the unknown sample.
UV-Vis Absorption Experiment 2: Beer Lambert Law & Determination of the Aromatic Label in a Synthetic Polymer
This experiment provides experience for students in quantitative analysis. UV-Vis spectra will be recorded for several, simple aromatic molecules in toluene solution. Beer Lambert plots will be constructed for each aromatic species and the molar extinction coefficient determined.
In the second part of the experiment, students are allocated polymer samples with unknown trace amounts of aromatic label. Given the information derived in part one, UV-Vis spectroscopy will be used quantitatively to determine the amount of each aromatic component present in the unknown samples.
UV-Vis Absorption Experiment 3: Absorption Spectrometer Simulator Containing Absorption Spectra of a Selection of Materials
Users can select from 25 solute/solvent combinations from a variety of materials including food dyes and transition metal complexes. This experiment provides experience of sample preparation using the virtual flask and setting up and preparing the absorption spectrometer simulator to run spectra from the virtual solutions. Users can select the wavelength range, scan speed and mode of operation (i.e., absorbance or transmittance) – It’s just like using the real thing!
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What do you get if you purchase UV-Vis Absorption Experiment 1: Beer Lambert Law & Identification of an Unknown Mixture?
UV-Vis Absorption Spectrometer Simulator; Teaching Notes (Sim Pack 4); Experimental Procedure; Teacher’s / Supervisor’s Notes including model answers.
Preview Documentation – Look Inside
Below are sample pages from our teaching documentation to give you a flavor for the quality of material supplied upon purchase of one of our experiments.
UV-Vis Absorption Experiment 1 Preview – Beer Lambert Law and Identification of an Unknown Mixture:
Our simulators do not require the latest or the greatest computers. If you have a new PC then great but if you need to use an obsolete PC running Window-98 SE or later then, provided it can read a CD you can probably use it. Just give it a try – you have nothing to lose!
What to do next?
Simply download the fully functional “demonstration version” of our software for evaluation purposes. If you wish to proceed to purchase a full licence for teaching purposes contact our sales department to arrange payment following which you will also receive additional documentation and other support material.